Fluidic Behavior of Polymer Compounds in an Open-Tubular Capillary with Ternary Mixed Carrier Solvents under Laminar Flow Conditions

An open-tubular capillary chromatography is developed based on tube radial distribution of the ternary mixed carrier solvents of water-hydrophilic/hydrophobic organic under laminar flow conditions. This is called “tube radial distribution chromatography” (TRDC). Fluidic behavior of the polymer compounds, FluoSpheres (1.0 m diameter), was examined by the TRDC system. The ternary mixture of water-acetonitrile-ethyl acetate, 15:3:2 (water-rich) or 3:8:4 (organic solvent-rich) volume ratio, as a carrier solution was fed into the fused-silica capillary tube under laminar flow conditions. The peak of the FluoSpheres appeared with the apex at the maximum linear velocity and the slope of the non-Gaussian peak did not last longer than the elution time at the average linear velocity with the water-rich carrier solution. With the organic solvent-rich carrier solution FluoSpheres was eluted at near the average linear velocity.